Quintara Long-Read Microbial WGS provides whole-genome sequencing for cultured bacterial and yeast samples using 30–50× Nanopore long-read coverage. The workflow generates long reads that can span repetitive regions, resolve structural variations, and characterize complex genomic features, making it well suited for de novo genome assembly and genome structure analysis.
Capture genome structure and resolve complex regions
Access methylation information from native DNA data
Get sequencing results in as fast as 1 day
Deliver comprehensive assembly, QC
What Is Nanopore Long-Read WGS Used For?
Create Build a genome from scratch | Resolve Reveal complex genome features | Characterize Understand overall genome architecture | ||
Create a de novo assembly without relying on a reference Assemble bacterial and yeast genomes from long-read data | Resolve repetitive regions and large structural variations Explore native-DNA methylation patterns | Analyze genome organization and complex genomic regions Compare genome structures across related isolates |
A streamlined workflow from DNA QC and Nanopore sequencing to de novo genome assembly and analysis, designed to generate long-read microbial genome insights.
Cultured bacterial or yeast isolates
DNA QC and Nanopore library preparation
30–50× Nanopore long-read sequencing
De novo assembly, genome annotation
Sequencing data, assembled genomes, and analysis results
Service | Technology & Coverage | Sample Type | Price | Turnaround Time |
Long-Read Microbial WGS | 30–50x Nanopore long-read | Bacterial | Starting from $90 | 1-2 Business Days |
Yeast | $150 |
* Your project clock starts once we receive your samples.
DNA Extraction — $15 per sample (optional)
Methylation Analysis — $30 per sample (optional)
1. Purified gDNA
Concentration: 50 ng/μL
Volume: ≥30 μL
2. Cell Pellet
Volume:1-2mL
Pellet yeast maintained at freezing (dry ice/Ice pack)
Sequencing & Assembly QC Report (HTML)
NanoPlot Sequencing QC
Bandage Assembly Visualization
CheckM Completeness & Contamination Assessment
Species Identification
De Novo Assembled & Annotated Consensus Genome
Raw Nanopore FASTQ Data
Methylation Analysis Report (optional)
Not necessarily. Coverage indicates how many times the genome is represented in the sequencing data, but it does not determine read length or assembly continuity. For de novo assembly, long reads can span repetitive and complex regions that very high-coverage short reads may still fail to connect.
Both workflows are designed for pure bacterial or yeast isolates and require high-quality genomic DNA. Mixed or contaminated samples may affect genome assembly and downstream analysis.
Yes. Organism type and expected genome size help determine sequencing requirements, data yield, and project pricing.
It means the total sequenced bases are roughly 30-50 times the expected genome size on average. Individual regions may receive more or less coverage.
No. Both Long-Read and Hybrid WGS can generate a de novo assembly without relying on a close reference genome. A suitable reference may be used for additional reference-based analyses when needed.
Choose Long-Read when the main goal is de novo assembly and genome structure, Hybrid when both assembly continuity and base-level confidence are important.
Yes. Additional analyses, including reference-based mapping, variant calling, and methylation analysis, may be available depending on project requirements and service availability. Methylation analysis requires native DNA.
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